nontumorigenic human breast epithelial cell line mcf10a (ATCC)
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nontumorigenic human breast epithelial cell line mcf10a
Nontumorigenic Human Breast Epithelial Cell Line Mcf10a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+epithelial+cell+line+mcf10a/MCF+10A/pm41899470-171-19-30
Average 99 stars, based on 8147 article reviews
Nontumorigenic Human Breast Epithelial Cell Line Mcf10a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+epithelial+cell+line+mcf10a/MCF+10A/pm41899470-171-19-30
Average 99 stars, based on 8147 article reviews
nontumorigenic human breast epithelial cell line mcf10a - by Bioz Stars,
2026-10
99/100 stars
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Recombinant:Article Title: Pleiotrophin modulates cell proliferation, prostate smooth muscle contraction and fibrosis in hyperplastic prostate. Article Snippet: .. Article Title: Pleiotrophin modulates cell proliferation, prostate smooth muscle contraction and fibrosis in hyperplastic prostate Article Snippet: .. Cell Culture:Article Title: Comprehensive analysis of metabolism-related gene biomarkers reveals their impact on the diagnosis and prognosis of triple-negative breast cancer. Article Snippet: .. The sequences of primers used were as follows: IDO1-F: 5’- G C A A A T G C A A G A A C G G G A C A-3’ and IDO1-R: 5’- A T A G C T G G G G G T T G C C T T T C-3’; SDS-F: 5’- C T G C C C A A G A T C A C C A G T G T-3’ and SDS-R: 5’- G C C T C C T G G T C C G A G A T A A C-3’; GLDC-F: 5’- G T A C A G C T C A G G C C C T C T T G-3’ and GLDC-R: T G C T C G C T T G A G A C C T T C T G. Cell culture and SiRNA transfection The breast cancer cell lines MDA-MB-231 and MCF-7, as well as the normal Transfection:Article Title: Comprehensive analysis of metabolism-related gene biomarkers reveals their impact on the diagnosis and prognosis of triple-negative breast cancer. Article Snippet: .. The sequences of primers used were as follows: IDO1-F: 5’- G C A A A T G C A A G A A C G G G A C A-3’ and IDO1-R: 5’- A T A G C T G G G G G T T G C C T T T C-3’; SDS-F: 5’- C T G C C C A A G A T C A C C A G T G T-3’ and SDS-R: 5’- G C C T C C T G G T C C G A G A T A A C-3’; GLDC-F: 5’- G T A C A G C T C A G G C C C T C T T G-3’ and GLDC-R: T G C T C G C T T G A G A C C T T C T G. Cell culture and SiRNA transfection The breast cancer cell lines MDA-MB-231 and MCF-7, as well as the normal Multiple Displacement Amplification:Article Title: Comprehensive analysis of metabolism-related gene biomarkers reveals their impact on the diagnosis and prognosis of triple-negative breast cancer. Article Snippet: .. The sequences of primers used were as follows: IDO1-F: 5’- G C A A A T G C A A G A A C G G G A C A-3’ and IDO1-R: 5’- A T A G C T G G G G G T T G C C T T T C-3’; SDS-F: 5’- C T G C C C A A G A T C A C C A G T G T-3’ and SDS-R: 5’- G C C T C C T G G T C C G A G A T A A C-3’; GLDC-F: 5’- G T A C A G C T C A G G C C C T C T T G-3’ and GLDC-R: T G C T C G C T T G A G A C C T T C T G. Cell culture and SiRNA transfection The breast cancer cell lines MDA-MB-231 and MCF-7, as well as the normal Article Title: Mechanism of LINC00958 in ferroptosis of breast cancer through the SRSF1/GPX4 axis. Article Snippet: All procedures involving the use, care, and handling of animals were complied with the National Institutes of Health Guide for the Care and Use of Laboratory Animals [21]. .. The human BC cell lines MDA-MB-157 ( h t t p s : / / w w w . c e l l o s a u r u s . o r g / C V C L _ 0 6 1 8; ER-/PR-/HER2-/Low TNBC subtype), MDA-MB-468 ( h t t p s : / / w w w . c e l l o s a u r u s . o r g / C V C L _ 0 4 1 9; ER−/PR−/HER2− TNBC subtype), MDAMB-231 ( h t t p s : / / w w w . c e l l o s a u r u s . o r g / C V C L _ 0 0 6 2; ER−/PR−/HER2− TNBC subtype) and the normal Article Title: Mechanism of LINC00958 in ferroptosis of breast cancer through the SRSF1/GPX4 axis Article Snippet: .. The human BC cell lines MDA-MB-157 ( https://www.cellosaurus.org/CVCL_0618 ; ER-/PR-/HER2-/Low TNBC subtype), MDA-MB-468 ( https://www.cellosaurus.org/CVCL_0419 ; ER−/PR−/HER2− TNBC subtype), MDA-MB-231 ( https://www.cellosaurus.org/CVCL_0062 ; ER−/PR−/HER2− TNBC subtype) and the normal Article Title: TRPM7–Annexin A1 Mechanosensitive Pathway Drives Capillary Infiltration by Circulating Tumor Cells Article Snippet: .. Human breast epithelial cancer cell lines, MCF7, and MDA-MB-231, and immortalized |
![Induction and characterization of EMT and MET in breast cancer cell lines. (A) Immunoblot analysis of EMT induction in MCF7 cells transiently overexpressing TWIST1-GFP for 48 h. Cells were transfected with 2 μg of pEGFPN1 or hTWIST1-GFP (B) Immunofluorescence analysis of EMT in MCF7 cells overexpressing TWIST1-GFP or control pEGFPN1. E-cadherin and Vimentin (red), nucleus (DAPI, blue). Scale bar ∼10 μm (C) Schematic representation of EMT induction in MCF7 cells (D) Scatter plot of immunofluorescence assay, showing relative changes in the integrated density of E-cadherin and Vimentin in MCF7 cells. Quantification for panel (B) ( n = 200). Data represent mean ± standard deviation (SD) from N = 3 independent biological replicates. (E) Immunoblot analysis of EMT induction in <t>MCF10A</t> cells treated with 10 ng/ml TGF-β for 7 days (F) Immunofluorescence analysis of EMT in MCF10A cells treated with TGF-β. E-cadherin (green); Vimentin (red); nucleus (blue, DAPI). Scale bar ∼10 μm (G) Schematic representation of EMT induction in MCF10A cells (H) Scatter plot of immunofluorescence assay, showing relative changes in the integrated density of E-cadherin and Vimentin in MCF10A cells [data shown in panel (F)] following EMT induction ( n = 200). Data represent mean ± SD from N = 3 independent biological replicates (I) Immunoblot analysis of MET induction in MDAMB231 cells following doxycycline-induced GRHL2 overexpression for 48 h (J) Immunofluorescence analysis of MET in MDAMB231 cells overexpressing GRHL2. E-cadherin and Vimentin (red), nucleus (blue, DAPI). Scale bar ∼10 μm (K) Schematic representation of MET induction in MDAMB231 cells (L) Scatter plot of immunofluorescence assay, showing relative changes in the integrated density of E-cadherin and Vimentin in MDAMB231 cells following MET induction [data shown in panel (J)] ( n = 200). Data represent mean ± SD from three independent biological replicates. Unpaired Student’s t -test was used to compute the P -value.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4643/pmc12774643/pmc12774643__gkaf1464fig1.jpg)
![Effect of Lamin A/C perturbation on EMT and MET. (A) Volcano plot showing differentially expressed genes in MCF10A cells upon Lamin A/C knockdown. Downregulated, upregulated, and nonsignificant genes. (B) Heatmap of the top 50 [ P <0.05; log 2 Fold Change (FC) > 2] differentially expressed genes in MCF10A cells upon Lamin A/C knockdown. Downregulated and upregulated genes, respectively ( N = 2 biological replicates). (C) GO enrichment analysis of differentially expressed genes ( P ≤0.05), showing the most enriched biological processes. (D) GSEA plot showing enrichment for EMT upon Lamin A/C knockdown (normalized enrichment score = 3.337). (E, F) Immunofluorescence analysis of MCF7 (E) and MCF10A (F) cells upon Lamin A/C knockdown. Lamin A/C, E-cadherin or Vimentin, and phalloidin. Nucleus (DAPI). Scale bars, ∼10 μm (G) Immunofluorescence analysis of MDAMB231 cells overexpressing Lamin A*-GFP upon endogenous Lamin A/C depletion. E-cadherin (top panel) or Vimentin (bottom panel), and Lamin A/C (−Dox and +Dox panels only). Nucleus (DAPI). Scale bar ∼10 μm. ( H–J ) Immunoblot analysis of EMT markers in MCF7 (H) , MCF10A (I) cells upon Lamin A/C knockdown, and MDAMB231 (J) cells upon Lamin A overexpression. RNA-Seq was performed in N = 2 independent biological replicates. Lamin A* denotes a full-length Lamin A construct resistant to doxycycline-induced depletion of endogenous Lamin A/C. Statistical significance, P -value <0.05.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4643/pmc12774643/pmc12774643__gkaf1464fig3.jpg)
![Dynamic Remodeling of the Lamin A/C Interactome During EMT and MET. (A, B) Venn diagrams showing unique and common interactors of Lamin A/C identified by (Immunoprecipitation - Mass Spectroscopy) IP-MS in MCF7 versus MCF7-TWIST1 (A) and MDAMB231 versus MDAMB231-GRHL2 (B). (C, D) Representative STRING network analysis of Lamin A/C interactors in MCF7 versus MCF7-TWIST1 (C) and MDAMB231 versus MDAMB231-GRHL2 (D) . ( E–G ) Co-IP of Lamin A/C in MCF7 (E) , MCF10A (F) , and MDAMB231 (G) cells upon EMT (E, F) or MET (G) induction, followed by immunoblotting for EZH2 and Lamin A/C. IgG: isotype control, an approximately equal amount of antibody is used for immunoprecipitation. (H, I) Proximity ligation assay (PLA) detects Lamin A/C–EZH2 interaction in MCF7 (H) and MDAMB231 (I) cells upon EMT (H) or MET (I) induction. PLA signal (red), nucleus (blue, DAPI). Scale bar: ∼10 μm. (J, K) Quantification of PLA signal in MCF7 (J) and MDAMB231 (K) cells. Data represent mean ± SD from N = 3, independent biological replicates, and P -values calculated by one-way ANOVA and means are compared between pBp-EV and pBP-Twist (J) and −Dox (GRHL2) and +Dox (GRHL2) (K). (L) Time-course analysis of Lamin A/C–EZH2 interaction by immunoprecipitation of Lamin A/C in MCF10A cells during EMT progression [∼12 to ∼168 h (∼7 days) post-TGF-β treatment] and MET recovery [5 days post-TGF-β withdrawal (WD)], assessed by Co-IP and immunoblotting. IgG: isotype control, statistical significance, P -value <0.05.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4643/pmc12774643/pmc12774643__gkaf1464fig4.jpg)
![Phosphorylation-dependent regulation of Lamin A/C–EZH2 binding in EMT and MET. (A, B) Schematic representation of the workflow for generating stable cell lines with inducible knockdown of Lamin A (A) or EZH2 (B) , followed by rescue with full-length, phosphodeficient, or phosphomimetic mutants. (C, D) Co-IP of Lamin A in MCF7 and MDAMB231 cells after doxycycline-induced Lamin A/C depletion and rescue with full-length, phosphodeficient (S22A), or phosphomimetic (S22D) Lamin A. TWIST1-GFP was transiently overexpressed in MCF7 cells, and GRHL2-GFP was stably overexpressed in MDAMB231 cells. (E, F) Coimmunoprecipitation of EZH2 in MCF7 and MDAMB231 cells after doxycycline-induced EZH2 depletion and rescue with full-length, phosphodeficient (T345A), or phosphomimetic (T345D) EZH2. TWIST1-GFP was transiently overexpressed in MCF7 cells, and GRHL2-GFP was stably overexpressed in MDAMB231 cells. (G) Immunofluorescence images of MCF10A cells showing the extent of colocalization between Lamin A [full-length, phosphodeficient (S22A), or phosphomimetic (S22D)] and EZH2 ± TGF-β. Nucleus (DAPI). Scale bar ∼10 μm. (H) Immunofluorescence images of MCF10A cells showing the extent of colocalization between EZH2 [full-length, phosphodeficient (T345A), or phosphomimetic (T345D)] nucleus (DAPI). Scale bar ∼10 μm. (I, J) Quantification of Lamin A and EZH2 colocalization in MCF10A cells using Mander’s coefficient. Unpaired Student’s t -test was used to compute the P -value. Means are compared between (I) LMNA-GFP (UT; control) versus LMNA-S22D (UT) and LMNA-GFP (TGFβ; control) versus LMNA-S22D (TGFβ). (J) EZH2-FLAG (UT; control) versus EZH2-T345D (UT) and EZH2-FLAG (TGFβ; control) versus EZH2-T345D (TGFβ). Statistical significance, P -value <0.05.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4643/pmc12774643/pmc12774643__gkaf1464fig6.jpg)
